Our ROC system ensures contaminant-free cultures throughout the entire life cycle, providing consistent and dependable results.
Transformed Root Organ Culture (t-ROC) Technology
“ Driving Arbuscular Mycorrhizal Fungi Cultivation to New Horizons ”
Transformed Root Organ Culture
Welcome to the world of Root Organ Culture (ROC) Technology - an innovative and advanced monoxenic culture system that revolutionizes the production of Vesicular Arbuscular Mycorrhizal (VAM) or Arbuscular Mycorrhizal Fungi (AMF). ROC shares similarities with Plant Tissue Culture (PTC) but offers distinct advantages for the efficient cultivation and propagation of these essential fungi.
Unraveling the ROC Method
ROC utilizes monoxenic culture, where VAM or AMF is cultivated in association with excised roots on a synthetic growth medium. It involves the development of hairy roots by modifying the plant root genotype using Ri-plasmid of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) bacteria. These hairy roots, along with Mycorrhizal fungi, coexist within plant tissue culture bottles, creating an advanced In Vitro system for AMF production.
Advantages of ROC System
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ROC technology enables the production of a large number of Mycorrhizal fungal spores within a relatively short period. This high spore yield ensures a consistent and ample supply of inoculants for agricultural applications.
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Morphological observations can be conducted without disturbing the intimate coexistence of host roots and AMF during the entire life cycle.
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ROC thrives under growth chamber controlled conditions, optimizing space for efficient production
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Important for large-scale production and in vitro propagation, ROC offers simplified quality control and quantification of fungal growth.
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ROC allows for non-destructive monitoring of Mycorrhizal fungi during the entire life cycle. Researchers can conduct morphological observations without disturbing the intimate coexistence of host roots and AMF, providing valuable insights into the symbiotic relationship.
Efficiency in Various Studies
Physiological Studies:
ROC's standardized culture conditions facilitate studies on the impact of environmental factors on Mycorrhizal fungi.
Molecular and Biochemical Studies:
Optimal conditions in ROC eliminate undesirable contaminants, making it ideal for conducting precise molecular and biochemical research
Mass-Inoculum Production:
Through regular subcultures, ROC enables the efficient production of mass-inoculum, supporting large-scale applications
